Gene Cloning: An Introduction |
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Page 95
... normal vector molecule Ampicillin resistance BamHI Figure 5.7 The cloning vector pBR322 . ( a ) The normal vector mole- cule . ( b ) A recombinant molecule containing an extra piece of DNA inserted into the BamHI site . For a more ...
... normal vector molecule Ampicillin resistance BamHI Figure 5.7 The cloning vector pBR322 . ( a ) The normal vector mole- cule . ( b ) A recombinant molecule containing an extra piece of DNA inserted into the BamHI site . For a more ...
Page 102
... normal phage plaques ( Figure 5.12 ( c ) ) . The end result of a gene cloning experiment using a λ or M13 vector is therefore an agar plate covered in phage plaques . Each plaque is derived from a single transfected or infected cell and ...
... normal phage plaques ( Figure 5.12 ( c ) ) . The end result of a gene cloning experiment using a λ or M13 vector is therefore an agar plate covered in phage plaques . Each plaque is derived from a single transfected or infected cell and ...
Page 225
... Normal gene Alul Alul Alul Alul ligase Deleted gene Normal protein Protein with a major deletion дро ( b ) Nucleotide removal at restriction site EcoRI EcoRI Small deletion ligase Bal31 nibbles ends Protein with a minor deletion зада Normal ...
... Normal gene Alul Alul Alul Alul ligase Deleted gene Normal protein Protein with a major deletion дро ( b ) Nucleotide removal at restriction site EcoRI EcoRI Small deletion ligase Bal31 nibbles ends Protein with a minor deletion зада Normal ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast