Gene Cloning: An Introduction |
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Page 175
... nucleotide sequence of the relevant gene . This prediction is always an approximation , as only methionine and ... nucleotides of the triplet coding for alanine can be predicted with certainty . As an example to clarify how these ...
... nucleotide sequence of the relevant gene . This prediction is always an approximation , as only methionine and ... nucleotides of the triplet coding for alanine can be predicted with certainty . As an example to clarify how these ...
Page 195
... nucleotide in the sequence is therefore A. The next most mobile band corresponds to a DNA molecule one nucleotide longer than the first . The track is noted , T in the exam- ple shown in Figure 9.8 ; the second nucleotide is therefore T ...
... nucleotide in the sequence is therefore A. The next most mobile band corresponds to a DNA molecule one nucleotide longer than the first . The track is noted , T in the exam- ple shown in Figure 9.8 ; the second nucleotide is therefore T ...
Page 316
... nucleotide that lacks the 3 ' hydroxyl group and so prevents further chain elongation when incorporated into a ... nucleotides in a DNA molecule . Double digestion Cleavage of a DNA molecule with two differ- ent restriction endonucleases ...
... nucleotide that lacks the 3 ' hydroxyl group and so prevents further chain elongation when incorporated into a ... nucleotides in a DNA molecule . Double digestion Cleavage of a DNA molecule with two differ- ent restriction endonucleases ...
Contents
Further reading | 12 |
Purification of DNA from living cells | 27 |
Further reading | 50 |
Copyright | |
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Common terms and phrases
able acid addition amount amplified analysis animal attached bacterial bacteriophage BamHI bands Biotechnology carried cDNA cell Chapter chromosome cloned gene cloning vectors coded coli colonies complete construction containing copy correct culture deletion desired detected developed direct disease DNA fragment DNA molecule EcoRI electrophoresis ends engineering enzyme example experiment expression Figure gene cloning genetic genome glycosylation host human hybridization identified important individual infection inserted involved labelled ligated marker means medium method molecular mRNA mutation natural needed normal nucleotide obtained occur organisms origin phage plant plaques plasmid polymerase position possible preparation present primers probe problem promoter protein purified reaction recombinant DNA molecule region removed replication resistance result segment selection separated sequence single single-stranded specific strand structure synthesis T-DNA techniques tion transcription transfer transformed translation types usually virus yeast