Gene Cloning: An Introduction |
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Page 160
... obtaining the clone you want Although there are many different procedures by which the desired clone can be obtained , all are variations on two basic themes . 1. Direct selection for the desired gene ( Figure 8.2 ( a ) ) , which means ...
... obtaining the clone you want Although there are many different procedures by which the desired clone can be obtained , all are variations on two basic themes . 1. Direct selection for the desired gene ( Figure 8.2 ( a ) ) , which means ...
Page 159
... obtain clones of specific genes and to provide information of importance to molecular biology and biotechnology . In this ... obtained , the molecular biologist is able to make use of a wide vari- ety of different techniques which will ...
... obtain clones of specific genes and to provide information of importance to molecular biology and biotechnology . In this ... obtained , the molecular biologist is able to make use of a wide vari- ety of different techniques which will ...
Page 160
... obtaining the clone you want Although there are many different procedures by which the desired clone can be obtained , all are variations on two basic themes . 1. Direct selection for the desired gene ( Figure 8.2 ( a ) ) , which means ...
... obtaining the clone you want Although there are many different procedures by which the desired clone can be obtained , all are variations on two basic themes . 1. Direct selection for the desired gene ( Figure 8.2 ( a ) ) , which means ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast