Gene Cloning: An Introduction |
From inside the book
Results 1-3 of 12
Page 97
... PUC8 . ( a ) The normal vector mole- cule . ( b ) A recombinant molecule containing an extra piece of DNA inserted into the BamHI site . For more detailed maps of pUC8 see Figures 6.3 and 6.4 . ( a ) pUC8 Ampicillin resistance gene O ...
... PUC8 . ( a ) The normal vector mole- cule . ( b ) A recombinant molecule containing an extra piece of DNA inserted into the BamHI site . For more detailed maps of pUC8 see Figures 6.3 and 6.4 . ( a ) pUC8 Ampicillin resistance gene O ...
Page 98
... pUC8 . ( a ) The bac- terial and plasmid genes comple- ment each other to produce a functional B - galactosidase ... pUC8 PUC8 Complete B - galactosidase molecules B - galactosidase fragment coded by bacterial gene • B - galactosidase ...
... pUC8 . ( a ) The bac- terial and plasmid genes comple- ment each other to produce a functional B - galactosidase ... pUC8 PUC8 Complete B - galactosidase molecules B - galactosidase fragment coded by bacterial gene • B - galactosidase ...
Page 112
... pUC8 . PUC8 has three important advantages that have led to it becom- ing one of the most popular E. coli cloning vectors . The first of these is fortuitous : the manipulations involved in construction of PUC8 were accompanied by a ...
... pUC8 . PUC8 has three important advantages that have led to it becom- ing one of the most popular E. coli cloning vectors . The first of these is fortuitous : the manipulations involved in construction of PUC8 were accompanied by a ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
13 other sections not shown
Other editions - View all
Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast