Gene Cloning: An Introduction |
From inside the book
Results 1-3 of 92
Page 27
... DNA that will be required is pure plasmid DNA . Preparation of plasmid DNA from a culture of bacteria fol- lows the same basic steps as purification of total cell DNA , with the crucial difference that at some stage the plasmid DNA must ...
... DNA that will be required is pure plasmid DNA . Preparation of plasmid DNA from a culture of bacteria fol- lows the same basic steps as purification of total cell DNA , with the crucial difference that at some stage the plasmid DNA must ...
Page 38
... plasmid DNA . These methods are based on the several physical differences between plasmid DNA and bacterial DNA , the most obvious of which is size . The largest plasmids are only 8 % of the size of the E. coli chromosome , and most are ...
... plasmid DNA . These methods are based on the several physical differences between plasmid DNA and bacterial DNA , the most obvious of which is size . The largest plasmids are only 8 % of the size of the E. coli chromosome , and most are ...
Page 43
An Introduction Terence A. Brown. Protein Linear and oc DNA Supercoiled DNA 1 1 1 ( a ) An EtBr - CsCI density gradient Buffer DNA RNA Remove supercoiled DNA with a syringe Figure 3.14 Purification of plasmid DNA by EtBr - CSCI density ...
An Introduction Terence A. Brown. Protein Linear and oc DNA Supercoiled DNA 1 1 1 ( a ) An EtBr - CsCI density gradient Buffer DNA RNA Remove supercoiled DNA with a syringe Figure 3.14 Purification of plasmid DNA by EtBr - CSCI density ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
13 other sections not shown
Other editions - View all
Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast