Gene Cloning: An Introduction |
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Page 258
... promoter can direct transcription . Strong promoters are those that can sustain a high rate of transcription ... promoter , so that the cloned gene is transcribed at the highest possible rate . ( a ) A strong promoter Gene Transcription ...
... promoter can direct transcription . Strong promoters are those that can sustain a high rate of transcription ... promoter , so that the cloned gene is transcribed at the highest possible rate . ( a ) A strong promoter Gene Transcription ...
Page 260
... promoter IPTG lacZ Transcription -35 -10 ( b ) The trp promoter 3 - ẞ - indoleacrylic Tryptophan acid trpA No Transcription- -35 -10 transcription ( c ) The tac promoter IPTG Transcription -35 -10 ( d ) λPL promoter < 30 ° C No ...
... promoter IPTG lacZ Transcription -35 -10 ( b ) The trp promoter 3 - ẞ - indoleacrylic Tryptophan acid trpA No Transcription- -35 -10 transcription ( c ) The tac promoter IPTG Transcription -35 -10 ( d ) λPL promoter < 30 ° C No ...
Page 334
... promoter 115 Sphaeroplasts 38 , 324g Spi phenotype 102 , 126 Staphylococcus aureus 63 , 63t Starch 270 Starch synthase 307t Stearyl - CoA desaturase 301t Stem cells 292 Stem - loop 255 , 324g Sticky end 22-3 , 64 , 324g Streptococcus 90 ...
... promoter 115 Sphaeroplasts 38 , 324g Spi phenotype 102 , 126 Staphylococcus aureus 63 , 63t Starch 270 Starch synthase 307t Stearyl - CoA desaturase 301t Stem cells 292 Stem - loop 255 , 324g Sticky end 22-3 , 64 , 324g Streptococcus 90 ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast