Gene Cloning: An Introduction |
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Page 258
... promoter can direct transcription . Strong promoters are those that can sustain a high rate of transcription ... promoter , so that the cloned gene is transcribed at the highest possible rate . ( a ) A strong promoter Gene Strong ...
... promoter can direct transcription . Strong promoters are those that can sustain a high rate of transcription ... promoter , so that the cloned gene is transcribed at the highest possible rate . ( a ) A strong promoter Gene Strong ...
Page 260
... promoter IPTG lacZ -35 -10 Transcription ( b ) The trp promoter 3 - ẞ - indoleacrylic Tryptophan acid trpA No Transcription- -35 -10 transcription ( c ) The tac promoter IPTG Transcription -35 -10 ( d ) λP ̧ promoter < 30 ° C No ...
... promoter IPTG lacZ -35 -10 Transcription ( b ) The trp promoter 3 - ẞ - indoleacrylic Tryptophan acid trpA No Transcription- -35 -10 transcription ( c ) The tac promoter IPTG Transcription -35 -10 ( d ) λP ̧ promoter < 30 ° C No ...
Page 334
... promoter 115 Sphaeroplasts 38 , 324g Spi phenotype 102 , 126 Staphylococcus aureus 63 , 63t Starch 270 Starch synthase 307t Stearyl - CoA desaturase 301t Stem cells 292 Stem - loop 255 , 324g Sticky end 22-3 , 64 , 324g Streptococcus 90 ...
... promoter 115 Sphaeroplasts 38 , 324g Spi phenotype 102 , 126 Staphylococcus aureus 63 , 63t Starch 270 Starch synthase 307t Stearyl - CoA desaturase 301t Stem cells 292 Stem - loop 255 , 324g Sticky end 22-3 , 64 , 324g Streptococcus 90 ...
Contents
Further reading | 12 |
Purification of DNA from living cells | 27 |
Further reading | 50 |
Copyright | |
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Common terms and phrases
able acid addition amount amplified analysis animal attached bacterial bacteriophage BamHI bands Biotechnology carried cDNA cell Chapter chromosome cloned gene cloning vectors coded coli colonies complete construction containing copy correct culture deletion desired detected developed direct disease DNA fragment DNA molecule EcoRI electrophoresis ends engineering enzyme example experiment expression Figure gene cloning genetic genome glycosylation host human hybridization identified important individual infection inserted involved labelled ligated marker means medium method molecular mRNA mutation natural needed normal nucleotide obtained occur organisms origin phage plant plaques plasmid polymerase position possible preparation present primers probe problem promoter protein purified reaction recombinant DNA molecule region removed replication resistance result segment selection separated sequence single single-stranded specific strand structure synthesis T-DNA techniques tion transcription transfer transformed translation types usually virus yeast