Gene Cloning: An Introduction |
From inside the book
Results 1-3 of 38
Page 10
... reaction ( PCR ) provides today's biologists with a second approach to gene isolation . In a PCR experiment a single ... reaction and its many appli- cations are covered in Chapter 11 . pyrF dnaL cysB trpE trpD trpC trpB trpA tonB opp ...
... reaction ( PCR ) provides today's biologists with a second approach to gene isolation . In a PCR experiment a single ... reaction and its many appli- cations are covered in Chapter 11 . pyrF dnaL cysB trpE trpD trpC trpB trpA tonB opp ...
Page 67
... reaction mixture . In our example , a suitable final volume for the reaction mixture would be 20 μl , so we add 2 μl of 10 x BglII buffer to the 16 μl of DNA already present ( Figure 4.11 ( b ) ) . The restriction endonuclease can now ...
... reaction mixture . In our example , a suitable final volume for the reaction mixture would be 20 μl , so we add 2 μl of 10 x BglII buffer to the 16 μl of DNA already present ( Figure 4.11 ( b ) ) . The restriction endonuclease can now ...
Page 195
An Introduction Terence A. Brown. ( c ) Four separate reactions result in four families of termi- nated strands The strand synthesis reaction is carried out four times in parallel . As well as the reaction with dideoxyATP , there will be ...
An Introduction Terence A. Brown. ( c ) Four separate reactions result in four families of termi- nated strands The strand synthesis reaction is carried out four times in parallel . As well as the reaction with dideoxyATP , there will be ...
Contents
Further reading | 12 |
Purification of DNA from living cells | 27 |
Further reading | 50 |
Copyright | |
18 other sections not shown
Other editions - View all
Common terms and phrases
able acid addition amount amplified analysis animal attached bacterial bacteriophage BamHI bands Biotechnology carried cDNA cell Chapter chromosome cloned gene cloning vectors coded coli colonies complete construction containing copy correct culture deletion desired detected developed direct disease DNA fragment DNA molecule EcoRI electrophoresis ends engineering enzyme example experiment expression Figure gene cloning genetic genome glycosylation host human hybridization identified important individual infection inserted involved labelled ligated marker means medium method molecular mRNA mutation natural needed normal nucleotide obtained occur organisms origin phage plant plaques plasmid polymerase position possible preparation present primers probe problem promoter protein purified reaction recombinant DNA molecule region removed replication resistance result segment selection separated sequence single single-stranded specific strand structure synthesis T-DNA techniques tion transcription transfer transformed translation types usually virus yeast