Gene Cloning: An Introduction |
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Page 87
... recombinant DNA molecule itself . Cloning serves two main purposes . Firstly , it allows a large number of recombinant DNA molecules to be produced from a limited amount of starting material . At the outset only a few nanograms of ...
... recombinant DNA molecule itself . Cloning serves two main purposes . Firstly , it allows a large number of recombinant DNA molecules to be produced from a limited amount of starting material . At the outset only a few nanograms of ...
Page 284
... recombinant vaccine has been approved for use in humans , and the possibility of using hamster cells to produce an even better vac- cine is being explored . ( b ) Live recombinant vaccines The use of live vaccinia virus as a vaccine for ...
... recombinant vaccine has been approved for use in humans , and the possibility of using hamster cells to produce an even better vac- cine is being explored . ( b ) Live recombinant vaccines The use of live vaccinia virus as a vaccine for ...
Page 333
... Recombinant 94 , 322g Recombinant DNA molecule , defi- nition 4 , 322g Recombinant DNA technology , definition 4 , 322g Recombinant insulin 275-8 , 282t Recombinant protein 322g animal cells 270-2 E. coli 255-67 eukaryotic hosts 268-72 ...
... Recombinant 94 , 322g Recombinant DNA molecule , defi- nition 4 , 322g Recombinant DNA technology , definition 4 , 322g Recombinant insulin 275-8 , 282t Recombinant protein 322g animal cells 270-2 E. coli 255-67 eukaryotic hosts 268-72 ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast