Gene Cloning: An Introduction |
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Page 87
... recombinant DNA molecules . The next step in a gene cloning experiment is to introduce these molecules into living cells , usually bacteria , which will then ... DNA fragment . 5 Figure 5.1 Cloning can supply large amounts of recombinant DNA.
... recombinant DNA molecules . The next step in a gene cloning experiment is to introduce these molecules into living cells , usually bacteria , which will then ... DNA fragment . 5 Figure 5.1 Cloning can supply large amounts of recombinant DNA.
Page 88
... DNA . Single cell containing multiple copies of a recombinant DNA molecule Growth to a colony Provides several μg of recombinant DNA Inoculate into 500ml of liquid broth , incubate for 18 hours Provides several mg of recombinant DNA ...
... DNA . Single cell containing multiple copies of a recombinant DNA molecule Growth to a colony Provides several μg of recombinant DNA Inoculate into 500ml of liquid broth , incubate for 18 hours Provides several mg of recombinant DNA ...
Page 322
... DNA labelling that utilizes ran- dom DNA hexamers which anneal to single - stranded DNA and act as primers for complementary strand synthesis by a suitable enzyme . Recombinant A transformed cell that contains a recombinant DNA molecule .
... DNA labelling that utilizes ran- dom DNA hexamers which anneal to single - stranded DNA and act as primers for complementary strand synthesis by a suitable enzyme . Recombinant A transformed cell that contains a recombinant DNA molecule .
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast