Gene Cloning: An Introduction |
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Page 213
... region of DNA that can bind a regulatory protein . It should therefore be possible to identify control sequences upstream of a cloned gene by searching the relevant region for protein - binding sites . There are two different approaches ...
... region of DNA that can bind a regulatory protein . It should therefore be possible to identify control sequences upstream of a cloned gene by searching the relevant region for protein - binding sites . There are two different approaches ...
Page 228
... region of a DNA molecule . Any region of any DNA molecule can be chosen , so long as the sequences at the borders of the region are known . The border sequences must be known because in order to carry out a PCR , two short ...
... region of a DNA molecule . Any region of any DNA molecule can be chosen , so long as the sequences at the borders of the region are known . The border sequences must be known because in order to carry out a PCR , two short ...
Page 289
... region in the left drawing ) . Additional map- ping experiments narrowed this down to a 600 - kb region flanked by two previously - mapped loci , D17S1321 and D17S1325 ( middle drawing ) . After examination of expressed sequences , a ...
... region in the left drawing ) . Additional map- ping experiments narrowed this down to a 600 - kb region flanked by two previously - mapped loci , D17S1321 and D17S1325 ( middle drawing ) . After examination of expressed sequences , a ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast