Gene Cloning: An Introduction |
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Page 61
... restriction endonucleases allow the molecular biologist to cut DNA molecules in the precise , reproducible manner required for gene cloning . The discovery of these enzymes , which led to ... endonuclease in Restriction endonucleases 61.
... restriction endonucleases allow the molecular biologist to cut DNA molecules in the precise , reproducible manner required for gene cloning . The discovery of these enzymes , which led to ... endonuclease in Restriction endonucleases 61.
Page 62
... Restriction endonuclease cannot bind to the recognition sequence 4.2.2 Type II restriction endonucleases cut DNA at specific nucleotide sequences The central feature of Type II restriction endonucleases ( which will be referred to ...
... Restriction endonuclease cannot bind to the recognition sequence 4.2.2 Type II restriction endonucleases cut DNA at specific nucleotide sequences The central feature of Type II restriction endonucleases ( which will be referred to ...
Page 65
... endonuclease in a DNA molecule of known length can be calcu- lated mathematically . A tetranucleotide sequence ( e.g. GATC ) should occur once every 44 = 256 nucleotides , and a hexanucleotide ( e.g. GGATCC ) ... Restriction endonucleases 65.
... endonuclease in a DNA molecule of known length can be calcu- lated mathematically . A tetranucleotide sequence ( e.g. GATC ) should occur once every 44 = 256 nucleotides , and a hexanucleotide ( e.g. GGATCC ) ... Restriction endonucleases 65.
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast