Gene Cloning: An Introduction |
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Page 75
... restriction endonucleases at once . It may be possible to perform a double digestion in one step , if both enzymes have similar requirements for pH , Mg2 + concentration , etc. Alternatively , the two digestions may have to be carried ...
... restriction endonucleases at once . It may be possible to perform a double digestion in one step , if both enzymes have similar requirements for pH , Mg2 + concentration , etc. Alternatively , the two digestions may have to be carried ...
Page 124
... restriction sites Even a deleted λ genome , with the non - essential region removed , has multiple recognition sites for most restriction endonucleases . This is a problem that is often encountered when a new vector is being developed ...
... restriction sites Even a deleted λ genome , with the non - essential region removed , has multiple recognition sites for most restriction endonucleases . This is a problem that is often encountered when a new vector is being developed ...
Page 241
... restriction sites . After PCR the amplified fragments are treated with the restriction endonuclease , which cuts each molecule within the primer sequence , leaving sticky - ended fragments that can be ligated efficiently into a standard ...
... restriction sites . After PCR the amplified fragments are treated with the restriction endonuclease , which cuts each molecule within the primer sequence , leaving sticky - ended fragments that can be ligated efficiently into a standard ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast