Gene Cloning: An Introduction |
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Page 201
... result in loss of a restriction site . The presence of the RFLP is then a direct indication that the gene is defective and that the individual may suffer from the resulting disease . Unfortunately , only a few genetic diseases can be ...
... result in loss of a restriction site . The presence of the RFLP is then a direct indication that the gene is defective and that the individual may suffer from the resulting disease . Unfortunately , only a few genetic diseases can be ...
Page 231
... results of PCRs with well - designed and poorly- designed primers . Lane 1 shows a single amplified fragment of the expected size , the result of a well- designed experiment . In Lane 2 there is no amplification product , suggesting ...
... results of PCRs with well - designed and poorly- designed primers . Lane 1 shows a single amplified fragment of the expected size , the result of a well- designed experiment . In Lane 2 there is no amplification product , suggesting ...
Page 322
... result of expression of a cloned gene . Recombination The exchange of DNA sequences between differ- ent molecules , occurring either naturally or as a result of DNA manipulation . Relaxed Refers to the non - supercoiled conformation of ...
... result of expression of a cloned gene . Recombination The exchange of DNA sequences between differ- ent molecules , occurring either naturally or as a result of DNA manipulation . Relaxed Refers to the non - supercoiled conformation of ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast