Gene Cloning: An Introduction |
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Page 25
An Introduction Terence A. Brown. ( a ) Injection of single - stranded DNA into the host cell , followed by synthesis of the second strand Pilus M13 particle injects DNA into cell Single - stranded DNA Figure 2.11 The M13 infection cycle ...
An Introduction Terence A. Brown. ( a ) Injection of single - stranded DNA into the host cell , followed by synthesis of the second strand Pilus M13 particle injects DNA into cell Single - stranded DNA Figure 2.11 The M13 infection cycle ...
Page 168
... DNA polymerase I , which synthesizes the sec- ond cDNA strand ( Figure 8.7 ( c ) ) , resulting in a double - stranded DNA fragment that can be ligated into a vector and cloned ( Figure 8.7 ( d ) ) . The resulting cDNA clones will be ...
... DNA polymerase I , which synthesizes the sec- ond cDNA strand ( Figure 8.7 ( c ) ) , resulting in a double - stranded DNA fragment that can be ligated into a vector and cloned ( Figure 8.7 ( d ) ) . The resulting cDNA clones will be ...
Page 209
... DNA - RNA hybrid involves a single - strand specific nuclease such as S1 ( p . 54 ) . This enzyme degrades single - stranded DNA or RNA polynucleotides , including single - stranded regions at the end of predominantly double- stranded ...
... DNA - RNA hybrid involves a single - strand specific nuclease such as S1 ( p . 54 ) . This enzyme degrades single - stranded DNA or RNA polynucleotides , including single - stranded regions at the end of predominantly double- stranded ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast