Gene Cloning: An Introduction |
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Page 64
An Introduction Terence A. Brown. Figure 4.9 The ends produced by cleavage of DNA with different restriction endonucleases . ( a ) A blunt end produced by Alul . ( b ) A sticky end produced by EcoRI . ( c ) The same sticky ends produced ...
An Introduction Terence A. Brown. Figure 4.9 The ends produced by cleavage of DNA with different restriction endonucleases . ( a ) A blunt end produced by Alul . ( b ) A sticky end produced by EcoRI . ( c ) The same sticky ends produced ...
Page 78
... blunt - ended mole- cules . ( b ) Ligation of sticky - ended molecules . ( a ) Ligating blunt ends ( b ) Ligating sticky ends ооо Discontinuities Transient base- paired structure DNA ligase seals the discontinuities 4.3.2 Sticky ends ...
... blunt - ended mole- cules . ( b ) Ligation of sticky - ended molecules . ( a ) Ligating blunt ends ( b ) Ligating sticky ends ооо Discontinuities Transient base- paired structure DNA ligase seals the discontinuities 4.3.2 Sticky ends ...
Page 79
An Introduction Terence A. Brown. 4.3.3 Putting sticky ends on to a blunt - ended molecule For the reasons detailed in the preceding section , compatible sticky ends are desirable on the DNA molecules to be ligated together in a gene ...
An Introduction Terence A. Brown. 4.3.3 Putting sticky ends on to a blunt - ended molecule For the reasons detailed in the preceding section , compatible sticky ends are desirable on the DNA molecules to be ligated together in a gene ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast