Gene Cloning: An Introduction |
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Page 24
... structure . As we shall see in Chapter 6 , the cleavage and packaging processes recognize just the cos sites and the DNA sequences to either side of them . Changing the structure of the internal regions of the λ genome , for example by ...
... structure . As we shall see in Chapter 6 , the cleavage and packaging processes recognize just the cos sites and the DNA sequences to either side of them . Changing the structure of the internal regions of the λ genome , for example by ...
Page 183
... structure of a cloned gene and how to use cloned genes to study the structure of a genome . The relevant methods fall into three categories : 1. Techniques for determining the location of a cloned gene within a larger DNA molecule . 2 ...
... structure of a cloned gene and how to use cloned genes to study the structure of a genome . The relevant methods fall into three categories : 1. Techniques for determining the location of a cloned gene within a larger DNA molecule . 2 ...
Page 200
... STRUCTURE Is it always necessary to obtain a nucleotide sequence in order to study the structure of a gene or a genome ? The answer is no , as less detailed , but nonetheless valuable information can also be obtained by a variety of ...
... STRUCTURE Is it always necessary to obtain a nucleotide sequence in order to study the structure of a gene or a genome ? The answer is no , as less detailed , but nonetheless valuable information can also be obtained by a variety of ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast