Gene Cloning: An Introduction |
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Page 100
... Synthesis of λ capsid proteins by E. coli strain SMR10 , which carries a λ phage that has defective cos sites . ( b ) Synthesis of incomplete sets of λ capsid proteins by E. coli strains BHB2688 and BHB2690 . ( c ) A mix- ture of cell ...
... Synthesis of λ capsid proteins by E. coli strain SMR10 , which carries a λ phage that has defective cos sites . ( b ) Synthesis of incomplete sets of λ capsid proteins by E. coli strains BHB2688 and BHB2690 . ( c ) A mix- ture of cell ...
Page 229
... synthesis ( Figure 11.2 ( c ) ) . The cycle of denaturation - hybridization - synthesis is repeated , usually 25-30 times , resulting in the eventual synthesis of several hundred million copies of the amplified DNA fragment ( Figure ...
... synthesis ( Figure 11.2 ( c ) ) . The cycle of denaturation - hybridization - synthesis is repeated , usually 25-30 times , resulting in the eventual synthesis of several hundred million copies of the amplified DNA fragment ( Figure ...
Page 275
... synthesis of artificial genes for the A and B chains followed by production of fusion proteins in E. coli , illustrates a number of the general techniques used in recombinant protein production . ( a ) Synthesis and expression of ...
... synthesis of artificial genes for the A and B chains followed by production of fusion proteins in E. coli , illustrates a number of the general techniques used in recombinant protein production . ( a ) Synthesis and expression of ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast