Gene Cloning: An Introduction |
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Page 17
... types of plasmid can therefore be assigned to different incompatibility groups on the basis of whether or not they can coexist , and plasmids from a single incompatibility group are often related to each other in various ways . The ...
... types of plasmid can therefore be assigned to different incompatibility groups on the basis of whether or not they can coexist , and plasmids from a single incompatibility group are often related to each other in various ways . The ...
Page 19
... types the entire infection cycle is completed very quickly , possibly in less than 20 minutes . This type of rapid infec- tion is called a lytic cycle , as release of the new phage particles is associated with lysis of the bacterial ...
... types the entire infection cycle is completed very quickly , possibly in less than 20 minutes . This type of rapid infec- tion is called a lytic cycle , as release of the new phage particles is associated with lysis of the bacterial ...
Page 167
... type . For example , the gene for gliadin , one of the nutritionally important proteins present in wheat , is expressed ... types of cell . The key to this method is the enzyme reverse transcriptase Identification of a clone from a gene ...
... type . For example , the gene for gliadin , one of the nutritionally important proteins present in wheat , is expressed ... types of cell . The key to this method is the enzyme reverse transcriptase Identification of a clone from a gene ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast