Molecular Cloning: A Laboratory Manual, Volume 1

Front Cover
Cold Spring Harbor Laboratory, 1989 - Eukaryotic cells - 676 pages

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User Review  - iayork - LibraryThing

the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review

LibraryThing Review

User Review  - iayork - LibraryThing

the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review

Contents

BOOK 1
iv
Plasmid Vectors
xi
Gel Electrophoresis of
xix
Plasmid Vectors 1
1-6
Extraction and Purification of Plasmid DNA 1
1-21
LARGESCALE PREPARATIONS OF PLASMID DNA 1
1-33
PURIFICATION OF PLASMID DNA 1
1-40
Strategies for Cloning in Plasmid Vectors 1
1-53
Amplification and Storage of Cosmid Libraries 3 44
3-44
Problems Commonly Encountered When Using Cosmids 3 54
3-54
The Biology of Filamentous Bacteriophages 4 3
4-3
BACTERIAL HOSTS FOR BACTERIOPHAGE M13 VECTORS 4 12
4-12
THE USES OF SINGLESTRANDED DNA GENERATED BY RECOMBINANTS
4-19
51
4-51
DNA METHYLATION 5 15
53
Methylation by Commonly Used Strains of E coli 5 15
5-5

DEPHOSPHORYLATION OF LINEARIZED PLASMID DNA 1
1-60
Preparation and Transformation of Competent E coli 1
1-74
SMALLSCALE PREPARATIONS OF PLASMID DNA 1 25
1-85
INSERTIONAL INACTIVATION 1
1-87
References 1
1-105
Vectors
2-2
Vectors 2 9
2-9
CHOOSING A BACTERIAL HOST FOR BACTERIOPHAGE I VECTORS 2 55
2-55
PREPARING STOCKS OF BACTERIOPHAGE FROM SINGLE PLAQUES 2 64
2-64
PURIFICATION OF BACTERIOPHAGE I 2 73
2-73
EXTRACTION OF BACTERIOPHAGE DNA 2 80
2-81
Identification and Analysis of Recombinants 2 108
2-108
RAPID ANALYSIS OF BACTERIOPHAGE A ISOLATES 2
2-118
Cloning in Cosmid Vectors 3 5
3-4
COSMID VECTORS FOR TRANSFECTION OF MAMMALIAN CELLS 3 18
3-19
Construction of Genomic DNA Libraries in Cosmid Vectors 3 27
3-27
Hybridization and Autoradiography 7 52
5-7
DIGESTING DNA WITH RESTRICTION ENZYMES 5 28
5-28
DNA POLYMERASES 5 35
5-35
DNADEPENDENT RNA POLYMERASES 5 58
5-58
73
5-73
DNAbinding Proteins 5 87
5-87
PREPARATION AND EXAMINATION OF AGAROSE GELS 6 9
6-9
RECOVERY AND PURIFICATION OF DNA FRACTIONATED ON AGAROSE
6-22
Polyacrylamide Gel Electrophoresis 6 36
6-36
DETECTION OF DNA IN POLYACRYLAMIDE GELS 6 44
6-44
60
6-60
ISOLATION OF RNAs 7 6
7-6
SELECTION OF POLYA RNA 7 26
7-26
Analysis of RNA 7 37
7-37
ANALYSIS OF RNA BY PRIMER EXTENSION 7 79
7-79

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