Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 87
Page 2-18
... BamHI 21230 EcoRI 19400 Smal 18560 Koni 17050 Kpnl 11930 Pvul 5510 BamHI 45360 Rend 44050 Bgill 43870 BamHI 43450 Saci 41890 Bgl 41830 Kpnl 39940 BamHI 39630 Hindill 39590 Sacl 39440 BamHI 38110 Smal 37030 Bgl 36970 Bgill 36320 Bgill ...
... BamHI 21230 EcoRI 19400 Smal 18560 Koni 17050 Kpnl 11930 Pvul 5510 BamHI 45360 Rend 44050 Bgill 43870 BamHI 43450 Saci 41890 Bgl 41830 Kpnl 39940 BamHI 39630 Hindill 39590 Sacl 39440 BamHI 38110 Smal 37030 Bgl 36970 Bgill 36320 Bgill ...
Page 2-28
... BamHI 39890 Smal 39170 EcoRI 38810 Bghl 38750 Bgl 38100 Bgl 37460 Hindill 36900 Hindill 35790 Pvul 35710 Bgfl 34500 BamHI 33500 Xhol 33240 San 32750 San 31750 EcoRI 31620 Smal 27980 BamHI 27480 Hindill 26250 Pvul 26100 EcoRI 25160 ...
... BamHI 39890 Smal 39170 EcoRI 38810 Bghl 38750 Bgl 38100 Bgl 37460 Hindill 36900 Hindill 35790 Pvul 35710 Bgfl 34500 BamHI 33500 Xhol 33240 San 32750 San 31750 EcoRI 31620 Smal 27980 BamHI 27480 Hindill 26250 Pvul 26100 EcoRI 25160 ...
Page 2-40
... BamHI Q 39890 Smal 39170 EcoRI 38810 Bgill 38750 Bghl - 16 181 38100 Bg 37460 Hindill 36900 Hindill 35790 Pvul 35710 Bgill 34500 BamHI 33500 Xhol 33240 Sal 32750 Sall 31750 EcoRI 31620 Smal Ea8.5 xis Ea22 exo bet Ju 401 314 194 || Ea47 ...
... BamHI Q 39890 Smal 39170 EcoRI 38810 Bgill 38750 Bghl - 16 181 38100 Bg 37460 Hindill 36900 Hindill 35790 Pvul 35710 Bgill 34500 BamHI 33500 Xhol 33240 Sal 32750 Sall 31750 EcoRI 31620 Smal Ea8.5 xis Ea22 exo bet Ju 401 314 194 || Ea47 ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml