Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 4
Page 5-9
... CCWGG Eael YGGCCR HpaI GTTAAC Bce 2431 GATC AorI CCWGG Acc III TCCGGA Bsa PI GATC Apy I CCWGG HindIII AAGCTT BspMII TCCGGA Bsp671 GATC Atu BI CCWGG MluI ACGCGT NruI TCGCGA BspAI GATC Atu II CCWGG SpeI ACTAGT Sbo131 TCGCGA BspPII GATC Bin SI ...
... CCWGG Eael YGGCCR HpaI GTTAAC Bce 2431 GATC AorI CCWGG Acc III TCCGGA Bsa PI GATC Apy I CCWGG HindIII AAGCTT BspMII TCCGGA Bsp671 GATC Atu BI CCWGG MluI ACGCGT NruI TCGCGA BspAI GATC Atu II CCWGG SpeI ACTAGT Sbo131 TCGCGA BspPII GATC Bin SI ...
Page 5-18
... CCWGG Cm5CWGG m5 CCWGG AquI CYCGRG ? GGTACC ? m5 CYCGRG * GGTACC Asu II TTCGAA TTm5 CGAA ? Atu CI TGATCA ? TGm6ATCA Aval CYCGRG Cm5 CCGGG m5 CYCGRG Ava II GGWCC ? Aval nicking occurs slowly in the unmethylated strand of the ...
... CCWGG Cm5CWGG m5 CCWGG AquI CYCGRG ? GGTACC ? m5 CYCGRG * GGTACC Asu II TTCGAA TTm5 CGAA ? Atu CI TGATCA ? TGm6ATCA Aval CYCGRG Cm5 CCGGG m5 CYCGRG Ava II GGWCC ? Aval nicking occurs slowly in the unmethylated strand of the ...
Page 5-22
... CCWGG GGmb m5 CGCC Cm5 CSGG Cm4CSGG m5 CCATGG m6A Gm6ATC Pfal GATC Gm6ATC ? Comments SalI GTCGAC ? GTCGAC GTm5 CGAC GGNC5C GGNm5Chm5C ? Cm3. MphI MspI CCWGG CCGG Gm6ATC Gm6ATC GGCGC5C Cm5CWGG m5CCGG hm5 Chm5 CGG C4CWGG CCm6AGG m4CCWGG ...
... CCWGG GGmb m5 CGCC Cm5 CSGG Cm4CSGG m5 CCATGG m6A Gm6ATC Pfal GATC Gm6ATC ? Comments SalI GTCGAC ? GTCGAC GTm5 CGAC GGNC5C GGNm5Chm5C ? Cm3. MphI MspI CCWGG CCGG Gm6ATC Gm6ATC GGCGC5C Cm5CWGG m5CCGG hm5 Chm5 CGG C4CWGG CCm6AGG m4CCWGG ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml