Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 66
Page 6-25
... EDTA ( pH 8.0 ) . Replace the EDTA with 0.5 N NaOH , and soak the membrane for a further 5 minutes . Wash the membrane six times in sterile water . The strips may be stored at 4 ° C in sterile water for several weeks after they have ...
... EDTA ( pH 8.0 ) . Replace the EDTA with 0.5 N NaOH , and soak the membrane for a further 5 minutes . Wash the membrane six times in sterile water . The strips may be stored at 4 ° C in sterile water for several weeks after they have ...
Page 6-55
... EDTA ( pH 8.0 ) , 0.01 M Tris Cl ( pH 7.6 ) . . 2. Resuspend the cells at a concentration of 3 × 101o cells / ml in 0.05 M EDTA ( pH 8.0 ) at 0 ° C . 3. Prepare an equal volume of low - melting - temperature agarose ( 1 % ) in L buffer ...
... EDTA ( pH 8.0 ) , 0.01 M Tris Cl ( pH 7.6 ) . . 2. Resuspend the cells at a concentration of 3 × 101o cells / ml in 0.05 M EDTA ( pH 8.0 ) at 0 ° C . 3. Prepare an equal volume of low - melting - temperature agarose ( 1 % ) in L buffer ...
Page 7-20
... EDTA solution is made in 100 - ml batches by dissolving 96.0 g of CsCl in 90 ml of 0.01 M EDTA ( pH 7.5 ) and adding diethyl pyrocarbonate ( DEPC ) to a final concentration of 0.1 % . Allow the solution to stand for 30 minutes , and ...
... EDTA solution is made in 100 - ml batches by dissolving 96.0 g of CsCl in 90 ml of 0.01 M EDTA ( pH 7.5 ) and adding diethyl pyrocarbonate ( DEPC ) to a final concentration of 0.1 % . Allow the solution to stand for 30 minutes , and ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml