Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 53
Page 1-34
... ( pH 8.0 ) 1 mM EDTA ( pH 8.0 ) 3. Collect the bacterial cells by centrifugation as described in step 1 . LYSIS BY BOILING This method ( adapted from Holmes and Quigley 1981 ) is designed to be used in conjunction with a subsequent ...
... ( pH 8.0 ) 1 mM EDTA ( pH 8.0 ) 3. Collect the bacterial cells by centrifugation as described in step 1 . LYSIS BY BOILING This method ( adapted from Holmes and Quigley 1981 ) is designed to be used in conjunction with a subsequent ...
Page 6-55
... EDTA ( pH 8.0 ) , 0.01 M Tris Cl ( pH 7.6 ) . . 2. Resuspend the cells at a concentration of 3 × 101o cells / ml in 0.05 M EDTA ( pH 8.0 ) at 0 ° C . 3. Prepare an equal volume of low - melting - temperature agarose ( 1 % ) in L buffer ...
... EDTA ( pH 8.0 ) , 0.01 M Tris Cl ( pH 7.6 ) . . 2. Resuspend the cells at a concentration of 3 × 101o cells / ml in 0.05 M EDTA ( pH 8.0 ) at 0 ° C . 3. Prepare an equal volume of low - melting - temperature agarose ( 1 % ) in L buffer ...
Page 7-10
... EDTA ( pH 8.0 ) , 0.5 % SDS to each 90 - mm plate of cells . Using a policeman , scrape the lysate into a 15 - ml disposable polypropylene tube ( Falcon 2053 or equivalent ) . 3. Rinse the plate with 2 ml of 0.1 M sodium acetate ( pH ...
... EDTA ( pH 8.0 ) , 0.5 % SDS to each 90 - mm plate of cells . Using a policeman , scrape the lysate into a 15 - ml disposable polypropylene tube ( Falcon 2053 or equivalent ) . 3. Rinse the plate with 2 ml of 0.1 M sodium acetate ( pH ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml