Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 87
Page 2-70
... Incubate overnight at 37 ° C with vigorous agitation ( 300 cycles / minute in a rotary shaker ) . 2. Read the OD 600 ... Incubate for 20 minutes at 37 ° C with intermittent shaking . 7. Add each infected aliquot to 500 ml of NZCYM ...
... Incubate overnight at 37 ° C with vigorous agitation ( 300 cycles / minute in a rotary shaker ) . 2. Read the OD 600 ... Incubate for 20 minutes at 37 ° C with intermittent shaking . 7. Add each infected aliquot to 500 ml of NZCYM ...
Page 2-72
... Incubate overnight at the appropriate temperature with vigorous agitation ( 300 cycles / minute in a rotary shaker ) ... Incubate at 37 ° C with vigorous shaking until the OD of the cultures reaches 0.5 ( 3-4 hours ) . 600 3. Inoculate ...
... Incubate overnight at the appropriate temperature with vigorous agitation ( 300 cycles / minute in a rotary shaker ) ... Incubate at 37 ° C with vigorous shaking until the OD of the cultures reaches 0.5 ( 3-4 hours ) . 600 3. Inoculate ...
Page 6-57
... Incubate the tubes for 30 minutes at 4 ° C . 3. Remove the buffer and replace it with 2-3 volumes of fresh 1 × restriction enzyme buffer . Add 30-50 units of the appropriate restriction enzyme to each tube , and incubate the tubes for ...
... Incubate the tubes for 30 minutes at 4 ° C . 3. Remove the buffer and replace it with 2-3 volumes of fresh 1 × restriction enzyme buffer . Add 30-50 units of the appropriate restriction enzyme to each tube , and incubate the tubes for ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml