Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-41
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. The Klenow fragment of E. coli DNA polymerase I can displace hybridized ... ( Klenow Fragment ) Enzymes Used in Molecular Cloning 5.41 Bacteriophage T4 DNA Polymerase 5 44.
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. The Klenow fragment of E. coli DNA polymerase I can displace hybridized ... ( Klenow Fragment ) Enzymes Used in Molecular Cloning 5.41 Bacteriophage T4 DNA Polymerase 5 44.
Page 5-41
... ( Klenow and Henningsen 1970 ) . The Klenow fragment of E. coli DNA polymerase I that is available today from commercial sources consists of a single polypeptide chain ( M = 76,000 ) produced by cleavage of intact DNA polymerase I with ...
... ( Klenow and Henningsen 1970 ) . The Klenow fragment of E. coli DNA polymerase I that is available today from commercial sources consists of a single polypeptide chain ( M = 76,000 ) produced by cleavage of intact DNA polymerase I with ...
Page 5-42
... Klenow Fragment Activity : 5 ' - 3 ' DNA polymerase Substrate : Single - stranded DNA template with a primer containing a free 3 ' - hydroxyl group . Reaction : DNAOH Klenow fragment of E. coli DNA polymerase I Mg ++ dATP , dTTP , dGTP ...
... Klenow Fragment Activity : 5 ' - 3 ' DNA polymerase Substrate : Single - stranded DNA template with a primer containing a free 3 ' - hydroxyl group . Reaction : DNAOH Klenow fragment of E. coli DNA polymerase I Mg ++ dATP , dTTP , dGTP ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml