Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 55
Page 5-33
... substrate that gives half - maximal activity ) . When using radioactively labeled substrates , calculate the concentration of substrate in the reaction from the specific activity provided by the manufacturer . If necessary , adjust the ...
... substrate that gives half - maximal activity ) . When using radioactively labeled substrates , calculate the concentration of substrate in the reaction from the specific activity provided by the manufacturer . If necessary , adjust the ...
Page 5-60
... substrate Km pH optimum Sulfhydryl requirement ++ Mg ** requirement Effect of ionic strength Bacteriophage T4 DNA ligase cohesive ends - DNA blunt ends - DNA nicks RNA : DNA hybrids 5 ' cohesive termini , 0.6 μΜ blunt ends , 50 μм nicks ...
... substrate Km pH optimum Sulfhydryl requirement ++ Mg ** requirement Effect of ionic strength Bacteriophage T4 DNA ligase cohesive ends - DNA blunt ends - DNA nicks RNA : DNA hybrids 5 ' cohesive termini , 0.6 μΜ blunt ends , 50 μм nicks ...
Page 5-63
... Substrate : Active on double - stranded DNA with complementary cohesive termini that base pair to bring together 3 ' - hydroxyl and 5 ' - phosphate termini . In addition , the enzyme is active on nicked DNA and active , albeit far less ...
... Substrate : Active on double - stranded DNA with complementary cohesive termini that base pair to bring together 3 ' - hydroxyl and 5 ' - phosphate termini . In addition , the enzyme is active on nicked DNA and active , albeit far less ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml