Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
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Page xxii
... cDNA Libraries Strategies for cDNA Cloning 8.3 PREPARATION OF mRNA FOR CDNA CLONING 8.3 Source of the mRNA 8.3 Integrity of the mRNA 8.4 Abundant mRNAs 8.6 Low - abundance mRNAs 8.6 Methods of Enrichment 8.6 FRACTIONATION OF mRNA BY ...
... cDNA Libraries Strategies for cDNA Cloning 8.3 PREPARATION OF mRNA FOR CDNA CLONING 8.3 Source of the mRNA 8.3 Integrity of the mRNA 8.4 Abundant mRNAs 8.6 Low - abundance mRNAs 8.6 Methods of Enrichment 8.6 FRACTIONATION OF mRNA BY ...
Page 7-79
... cDNA complementary to the RNA template . The length of the resulting end - labeled cDNA , as measured by electrophoresis through a polyacrylamide gel under denaturing conditions , reflects the distance between the end - labeled nu ...
... cDNA complementary to the RNA template . The length of the resulting end - labeled cDNA , as measured by electrophoresis through a polyacrylamide gel under denaturing conditions , reflects the distance between the end - labeled nu ...
Page
... cDNA synthesis and cloning alternative methods mRNA - cDNA cloning , 8.27 sequential addition of different link- ers , 8.27-8.28 in single - stranded vectors , 8.32 amplification of libraries constructed in λgt10 , 8.78 constructed in ...
... cDNA synthesis and cloning alternative methods mRNA - cDNA cloning , 8.27 sequential addition of different link- ers , 8.27-8.28 in single - stranded vectors , 8.32 amplification of libraries constructed in λgt10 , 8.78 constructed in ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml