Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 91
Page 4-13
... carry an amber mutation . This requirement has long since been dropped , and most vectors in common use today do not carry such mutations . However , many of the strains of E. coli that are used as hosts were developed when the ...
... carry an amber mutation . This requirement has long since been dropped , and most vectors in common use today do not carry such mutations . However , many of the strains of E. coli that are used as hosts were developed when the ...
Page 4-16
... carry deleted or rearranged versions of the foreign sequences . Instead , we recommend propagating the foreign DNA in plasmid vectors that carry an origin of replication derived from a filamentous bac- teriophage ( phagemids ) ...
... carry deleted or rearranged versions of the foreign sequences . Instead , we recommend propagating the foreign DNA in plasmid vectors that carry an origin of replication derived from a filamentous bac- teriophage ( phagemids ) ...
Page 5-36
... carry out this reaction , since it alone has a 5 ' - 3 ' exonuclease activity that can remove nucleotides from the DNA strand ahead of the advancing enzyme . 2. The holoenzyme was originally used for synthesis of the second strand of ...
... carry out this reaction , since it alone has a 5 ' - 3 ' exonuclease activity that can remove nucleotides from the DNA strand ahead of the advancing enzyme . 2. The holoenzyme was originally used for synthesis of the second strand of ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml