Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-85
... cohesive termini of bacteriophage λ DNA to reanneal . Analyze an aliquot ( 0.2 μg ) by electrophoresis through a 0.5 ... cohesive termini causes the arms to increase in size . The annealed arms migrate through the 0.5 % agarose gel at a ...
... cohesive termini of bacteriophage λ DNA to reanneal . Analyze an aliquot ( 0.2 μg ) by electrophoresis through a 0.5 ... cohesive termini causes the arms to increase in size . The annealed arms migrate through the 0.5 % agarose gel at a ...
Page 5-10
... Cohesive Termini If the restriction enzyme cleaves each strand of the substrate DNA on the 5 ' side of the axis of dyad symmetry , the resulting staggered break yields fragments of DNA that carry protruding cohesive 5 ' termini . For ...
... Cohesive Termini If the restriction enzyme cleaves each strand of the substrate DNA on the 5 ' side of the axis of dyad symmetry , the resulting staggered break yields fragments of DNA that carry protruding cohesive 5 ' termini . For ...
Page 5-13
... cohesive termini whose first nucleotide is complementary to the fourth nucleotide and whose second nucleotide is complementary to the third nucleotide . Ligation of mismatched , partially repaired termini allows as many as 70 % of the ...
... cohesive termini whose first nucleotide is complementary to the fourth nucleotide and whose second nucleotide is complementary to the third nucleotide . Ligation of mismatched , partially repaired termini allows as many as 70 % of the ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml