Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 61
Page 1-105
... Construction and characteriza- tion of new cloning vehicles . II . A mul- tipurpose cloning system . Gene 2:95 . Burns , D.M. and I.R. Beacham . 1984 . Positive selection vectors : A small plas- mid vector useful for the direct selec ...
... Construction and characteriza- tion of new cloning vehicles . II . A mul- tipurpose cloning system . Gene 2:95 . Burns , D.M. and I.R. Beacham . 1984 . Positive selection vectors : A small plas- mid vector useful for the direct selec ...
Page 1-110
... Construction of plasmids containing the coding sequences . Science 196 : 1313 . Vernet , T. , P.C.K. Lau , S.A. Narang , and L.P. Visentin . 1985. A direct - selection vector derived from pColE3 - CA38 and adapted for foreign gene ...
... Construction of plasmids containing the coding sequences . Science 196 : 1313 . Vernet , T. , P.C.K. Lau , S.A. Narang , and L.P. Visentin . 1985. A direct - selection vector derived from pColE3 - CA38 and adapted for foreign gene ...
Page 3-27
... construction of large cosmid libraries when limited amounts of genomic DNA are available . CLONING IN PHOSPHATASE - TREATED COSMID VECTORS The basic steps Cosmid Vectors 3.27 Construction of Genomic DNA Libraries in Cosmid Vectors 3 27.
... construction of large cosmid libraries when limited amounts of genomic DNA are available . CLONING IN PHOSPHATASE - TREATED COSMID VECTORS The basic steps Cosmid Vectors 3.27 Construction of Genomic DNA Libraries in Cosmid Vectors 3 27.
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml