Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 85
Page 4-22
... ( containing , e.g. , agar or agarose ) , the diffusion of the progeny particles is limited . Filamentous bacteriophages such as M13 do not kill the cells they infect , but they do slow their growth approximately twofold . Under these ...
... ( containing , e.g. , agar or agarose ) , the diffusion of the progeny particles is limited . Filamentous bacteriophages such as M13 do not kill the cells they infect , but they do slow their growth approximately twofold . Under these ...
Page 4-48
... containing pUC118 or pUC119 or their derivatives in a sterile 15 - ml culture tube containing 2-3 ml of 2 × YT medium . Add M13KO7 to a final concentration of 2 × 107 pfu / ml . Incu- bate for 1.0-1.5 hours at 37 ° C with strong ...
... containing pUC118 or pUC119 or their derivatives in a sterile 15 - ml culture tube containing 2-3 ml of 2 × YT medium . Add M13KO7 to a final concentration of 2 × 107 pfu / ml . Incu- bate for 1.0-1.5 hours at 37 ° C with strong ...
Page 7-43
... Containing Formaldehyde This method is adapted from those of Lehrach et al . ( 1977 ) , Goldberg ( 1980 ) , and Seed ( 1982a ) . Caution : Formaldehyde vapors are toxic . Solutions containing formal- dehyde should be prepared in a ...
... Containing Formaldehyde This method is adapted from those of Lehrach et al . ( 1977 ) , Goldberg ( 1980 ) , and Seed ( 1982a ) . Caution : Formaldehyde vapors are toxic . Solutions containing formal- dehyde should be prepared in a ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml