Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 3-5
... vector DNA in vitro under conditions that favor the formation of the type of structure shown in Figure 3.1 . Here , the segment of foreign DNA is flanked by cosmid molecules and the two cos sites are arranged in the ... Cosmid Vectors 3.5.
... vector DNA in vitro under conditions that favor the formation of the type of structure shown in Figure 3.1 . Here , the segment of foreign DNA is flanked by cosmid molecules and the two cos sites are arranged in the ... Cosmid Vectors 3.5.
Page 3-7
... vector ( Bates and Swift 1983 ; Poustka et al . 1984 ) . As discussed later , the presence of these two sites greatly simplifies directional cloning in cosmid vectors ( see Figure 3.3 ) . • The construction of cosmid ... Cosmid Vectors 3.7.
... vector ( Bates and Swift 1983 ; Poustka et al . 1984 ) . As discussed later , the presence of these two sites greatly simplifies directional cloning in cosmid vectors ( see Figure 3.3 ) . • The construction of cosmid ... Cosmid Vectors 3.7.
Page 3-27
... vector DNA to form concatemers that can be packaged into bacteriophage A particles . As discussed previously , libraries constructed in bacteriophage ... Cosmid Vectors 3.27 Construction of Genomic DNA Libraries in Cosmid Vectors 3 27.
... vector DNA to form concatemers that can be packaged into bacteriophage A particles . As discussed previously , libraries constructed in bacteriophage ... Cosmid Vectors 3.27 Construction of Genomic DNA Libraries in Cosmid Vectors 3 27.
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml