Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 96
Page 1-33
... culture ) with strains of E. coli harboring high - copy - number plasmids carrying the pMB1 or ColE1 replicon . 1. Grow a 30 - ml culture of the bacterial strain carrying the plasmid of interest to late log phase ( i.e. , to an OD 600 ...
... culture ) with strains of E. coli harboring high - copy - number plasmids carrying the pMB1 or ColE1 replicon . 1. Grow a 30 - ml culture of the bacterial strain carrying the plasmid of interest to late log phase ( i.e. , to an OD 600 ...
Page 2-70
... culture of infected bacteria gives the best results ( Blattner et al . 1977 ; Maniatis et al . 1978 ) . 1. Place 100 ml of NZCYM in a 500 - ml flask and inoculate with a single colony of an appropriate bacterial host . Incubate ...
... culture of infected bacteria gives the best results ( Blattner et al . 1977 ; Maniatis et al . 1978 ) . 1. Place 100 ml of NZCYM in a 500 - ml flask and inoculate with a single colony of an appropriate bacterial host . Incubate ...
Page 4-48
... culture tube containing 2-3 ml of 2 × YT medium . Add M13KO7 to a final concentration of 2 × 107 pfu / ml . Incu- bate for 1.0-1.5 hours at 37 ° C with strong agitation ( 300 cycles / minute ) . The bacterial culture should be only ...
... culture tube containing 2-3 ml of 2 × YT medium . Add M13KO7 to a final concentration of 2 × 107 pfu / ml . Incu- bate for 1.0-1.5 hours at 37 ° C with strong agitation ( 300 cycles / minute ) . The bacterial culture should be only ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml