Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-83
... digestion products are present . 5. If digestion is incomplete , warm the reaction to the appropriate tempera- ture , add more restriction enzyme ( 50-100 units ) , and continue the incubation at the optimal temperature recommended by ...
... digestion products are present . 5. If digestion is incomplete , warm the reaction to the appropriate tempera- ture , add more restriction enzyme ( 50-100 units ) , and continue the incubation at the optimal temperature recommended by ...
Page 2-92
... Digestion of such a vector with both BamHI and EcoRI yields left and right arms that carry BamHI termini , a stuffer fragment carrying EcoRI termini , and short segments of the polycloning sites carrying EcoRI and BamHI termini . These ...
... Digestion of such a vector with both BamHI and EcoRI yields left and right arms that carry BamHI termini , a stuffer fragment carrying EcoRI termini , and short segments of the polycloning sites carrying EcoRI and BamHI termini . These ...
Page
... digestion with restriction en- zymes , 3.32 , 9.24-9.29 partial filling of recessed 3 ' termini of fragments , 9.29-9.30 pulsed - field gel electrophoresis , 6.50- 6.52 DNA preparation , 6.53-6.57 Southern hybridization analysis . See ...
... digestion with restriction en- zymes , 3.32 , 9.24-9.29 partial filling of recessed 3 ' termini of fragments , 9.29-9.30 pulsed - field gel electrophoresis , 6.50- 6.52 DNA preparation , 6.53-6.57 Southern hybridization analysis . See ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml