Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-44
... double - stranded DNA . pUC118 and pUC119 are deriva- tives of pUC18 and pUC19 that contain a 476 - bp fragment derived from the intergenic region of wild - type bacteriophage M13 ( from the HgiAI site at nucleotide 5465 to the Dral ...
... double - stranded DNA . pUC118 and pUC119 are deriva- tives of pUC18 and pUC19 that contain a 476 - bp fragment derived from the intergenic region of wild - type bacteriophage M13 ( from the HgiAI site at nucleotide 5465 to the Dral ...
Page 5-74
... double - stranded DNA is given by the equation : dM t = -2VM , n m dt [ Km + ( S ) , ] t n m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , V = maximum reaction velocity ( expressed as moles ...
... double - stranded DNA is given by the equation : dM t = -2VM , n m dt [ Km + ( S ) , ] t n m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , V = maximum reaction velocity ( expressed as moles ...
Page 7-58
... double - stranded DNA is denatured and incubated with mRNA under these conditions , those segments of DNA ( exons ) that are transcribed into mRNA form hybrids and the remainder of the DNA remains single - stranded . DNA that has not ...
... double - stranded DNA is denatured and incubated with mRNA under these conditions , those segments of DNA ( exons ) that are transcribed into mRNA form hybrids and the remainder of the DNA remains single - stranded . DNA that has not ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml