Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-74
... efficiency of transformation of different bacterial strains by plasmids . Bac- teria treated according to the original protocol of Mandel and Higa yield 105-106 transformed colonies / μg of supercoiled plasmid DNA . This efficiency can ...
... efficiency of transformation of different bacterial strains by plasmids . Bac- teria treated according to the original protocol of Mandel and Higa yield 105-106 transformed colonies / μg of supercoiled plasmid DNA . This efficiency can ...
Page 1-75
... efficiencies . The efficiency of transformation with linear bacteriophage à DNA molecules is only 0.1 % of that obtained with small supercoiled plasmid DNA . A two- to tenfold variation in transforma- tion efficiency has been observed ...
... efficiencies . The efficiency of transformation with linear bacteriophage à DNA molecules is only 0.1 % of that obtained with small supercoiled plasmid DNA . A two- to tenfold variation in transforma- tion efficiency has been observed ...
Page 1-76
... efficiency of bacterial transformation , it is best to set aside a batch of glassware that is used for no other purpose than to prepare competent bacteria . This glassware should be washed and rinsed by hand , filled with pure water ...
... efficiency of bacterial transformation , it is best to set aside a batch of glassware that is used for no other purpose than to prepare competent bacteria . This glassware should be washed and rinsed by hand , filled with pure water ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml