Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 66
Page 1-48
... ethanol to drain away . 7. Wash the pellet with 70 % ethanol at 4 ° C , and recentrifuge as in step 5 . Remove the supernatant as completely as possible , and store the open tube for a few minutes in an inverted position on a pad of ...
... ethanol to drain away . 7. Wash the pellet with 70 % ethanol at 4 ° C , and recentrifuge as in step 5 . Remove the supernatant as completely as possible , and store the open tube for a few minutes in an inverted position on a pad of ...
Page 6-26
... ethanol at 4 ° C . Store the mixture for 10 minutes at room temperature , and recover the DNA by centrifugation at 12,000g for 20 minutes at room temperature in a microfuge . Carefully rinse the pellet with 70 % ethanol , and then ...
... ethanol at 4 ° C . Store the mixture for 10 minutes at room temperature , and recover the DNA by centrifugation at 12,000g for 20 minutes at room temperature in a microfuge . Carefully rinse the pellet with 70 % ethanol , and then ...
Page 7-62
... ethanol . Store the solution at 0 ° C for at least 30 minutes . Recover the nucleic acids by centrifuga- tion at 12,000g for 15 minutes at 4 ° C in a microfuge . Discard the supernatant , wash the pellet with 70 % ethanol , and ...
... ethanol . Store the solution at 0 ° C for at least 30 minutes . Recover the nucleic acids by centrifuga- tion at 12,000g for 15 minutes at 4 ° C in a microfuge . Discard the supernatant , wash the pellet with 70 % ethanol , and ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml