Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-42
... ethidium bromide ( 10 mg / ml in water ) for every 10 ml of the DNA / CsCl solution . Immediately mix the ethidium bromide solution ( which floats on the surface ) with the denser DNA / CsCl solution . The final density of the solution ...
... ethidium bromide ( 10 mg / ml in water ) for every 10 ml of the DNA / CsCl solution . Immediately mix the ethidium bromide solution ( which floats on the surface ) with the denser DNA / CsCl solution . The final density of the solution ...
Page 1-49
... Ethidium bromide is a powerful mutagen and is moderately toxic . After use , solutions containing this dye should be decontaminated by one of ... Ethidium Bromide Solutions 1 DECONTAMINATION OF CONCENTRATED SOLUTIONS OF ETHIDIUM BROMIDE 1.
... Ethidium bromide is a powerful mutagen and is moderately toxic . After use , solutions containing this dye should be decontaminated by one of ... Ethidium Bromide Solutions 1 DECONTAMINATION OF CONCENTRATED SOLUTIONS OF ETHIDIUM BROMIDE 1.
Page 6-15
... ethidium bromide : DNA complexes is much greater than that of unbound dye , small amounts of DNA can be detected in the presence of free ethidium bromide in the gel . Ethidium bromide can be used to detect both single- and double ...
... ethidium bromide : DNA complexes is much greater than that of unbound dye , small amounts of DNA can be detected in the presence of free ethidium bromide in the gel . Ethidium bromide can be used to detect both single- and double ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml