Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 95
Page 1-53
... DNA is cleaved with a restriction enzyme and joined in vitro to foreign DNA . The resulting recombinant plasmids are then used to transform bacteria . In practice , however , the plasmid vector must be carefully chosen to minimize the ...
... DNA is cleaved with a restriction enzyme and joined in vitro to foreign DNA . The resulting recombinant plasmids are then used to transform bacteria . In practice , however , the plasmid vector must be carefully chosen to minimize the ...
Page 1-54
... Foreign DNA to Plasmid Vectors Termini carried by fragment of foreign DNA Blunt - ended Different protruding termini Identical protruding termini Requirements for cloning high concentrations of DNAs and ligase for maximum efficiency ...
... Foreign DNA to Plasmid Vectors Termini carried by fragment of foreign DNA Blunt - ended Different protruding termini Identical protruding termini Requirements for cloning high concentrations of DNAs and ligase for maximum efficiency ...
Page 4-33
... foreign DNA are inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a ... DNA is ligated with an excess of foreign DNA that has compatible termini . No effort is made to reduce the background ...
... foreign DNA are inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a ... DNA is ligated with an excess of foreign DNA that has compatible termini . No effort is made to reduce the background ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml