Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 81
Page 2-57
... growth of recВ mutants ( Kushner et al . 1974 ) . A bacterial gene that encodes exonuclease I , an enzyme that is part of the recF pathway . The sbcB mutation improves the growth of recB and recC mutants ( Kushner et al . 1971 ; Leach ...
... growth of recВ mutants ( Kushner et al . 1974 ) . A bacterial gene that encodes exonuclease I , an enzyme that is part of the recF pathway . The sbcB mutation improves the growth of recB and recC mutants ( Kushner et al . 1971 ; Leach ...
Page 2-58
... growth of Spi bacteriophage Will modify but not restrict DNA For growth of Spi bacteriophage Will modify but not restrict DNA For growth of Spi bacteriophage References Wyman and Wertman ( 1987 ) Nader et al . ( 1985 ) Arber et al ...
... growth of Spi bacteriophage Will modify but not restrict DNA For growth of Spi bacteriophage Will modify but not restrict DNA For growth of Spi bacteriophage References Wyman and Wertman ( 1987 ) Nader et al . ( 1985 ) Arber et al ...
Page 2-66
... growth on the infected plates is due to lysis or inadequate growth of the host . 3. Incubate the plate without inversion for 6-8 hours at 37 ° C . Plates are not inverted during incubation to prevent the top agar / agarose from slipping ...
... growth on the infected plates is due to lysis or inadequate growth of the host . 3. Incubate the plate without inversion for 6-8 hours at 37 ° C . Plates are not inverted during incubation to prevent the top agar / agarose from slipping ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml