Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-25
... infected bacteria do not lyse , but instead grow slowly to form a dilute suspension . The inoculum for the ... infected culture for either 4-5 hours ( entire plaque ) or 5-6 hours ( one tenth of a plaque suspension ) at 37 ° C with ...
... infected bacteria do not lyse , but instead grow slowly to form a dilute suspension . The inoculum for the ... infected culture for either 4-5 hours ( entire plaque ) or 5-6 hours ( one tenth of a plaque suspension ) at 37 ° C with ...
Page 4-26
... infected bacteria by the same methods used to prepare plasmid DNA . Small - scale Preparations of the Replicative Form of Bacteriophage M13 DNA To 1. Prepare an infected culture ( 2 ml ) as described on page 4.25 . minimize the ...
... infected bacteria by the same methods used to prepare plasmid DNA . Small - scale Preparations of the Replicative Form of Bacteriophage M13 DNA To 1. Prepare an infected culture ( 2 ml ) as described on page 4.25 . minimize the ...
Page 4-29
... infected culture is used to prepare single - stranded DNA . 2. Transfer 1.0–1.5 ml of the infected culture to a microfuge tube , and centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . 3. Transfer about 1.2-1.3 ml of the ...
... infected culture is used to prepare single - stranded DNA . 2. Transfer 1.0–1.5 ml of the infected culture to a microfuge tube , and centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . 3. Transfer about 1.2-1.3 ml of the ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml