Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 30
Page 5-50
... interest , and the second is complementary to the other strand of the tem- plate downstream from the region of interest . The denatured template is incu- bated in the presence of a vast molar excess of both primers to generate primer ...
... interest , and the second is complementary to the other strand of the tem- plate downstream from the region of interest . The denatured template is incu- bated in the presence of a vast molar excess of both primers to generate primer ...
Page 5-58
... interest under the control of the bacteriophage T7 promoter are then introduced into the lysogens containing the bacteriophage T7 RNA polymerase gene . Activation of the bacteriophage T7 promoter is then achieved by isopropylthio - B ...
... interest under the control of the bacteriophage T7 promoter are then introduced into the lysogens containing the bacteriophage T7 RNA polymerase gene . Activation of the bacteriophage T7 promoter is then achieved by isopropylthio - B ...
Page 7-37
... interest is then located by hybridization with radiolabeled DNA or RNA followed by autoradiography . • Dot and slot hybridization , in which an excess of radiolabeled probe is hybridized to RNA that has been immobilized on a solid ...
... interest is then located by hybridization with radiolabeled DNA or RNA followed by autoradiography . • Dot and slot hybridization , in which an excess of radiolabeled probe is hybridized to RNA that has been immobilized on a solid ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml