Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-20
... Isoschizomers of DpnI include Cful , NanII , NmuDI , NmuEI , and NsuDI . DpnI cuts dam - modified bacteriophage XP12 DNA . FokI cuts about two- to fourfold more slowly at CATCC than at unmodified sites . Haell gives various rate effects ...
... Isoschizomers of DpnI include Cful , NanII , NmuDI , NmuEI , and NsuDI . DpnI cuts dam - modified bacteriophage XP12 DNA . FokI cuts about two- to fourfold more slowly at CATCC than at unmodified sites . Haell gives various rate effects ...
Page 5-94
... isoschizomers . Nucleic Acids Res . ( suppl . ) 16 : r271 . Roberts , T.M. , S.L. Swanberg , A. Poteete , G. Riedel , and K. Backman . 1980. A plasmid cloning vehicle allowing a positive selection for inserted frag- ments . Gene 12 ...
... isoschizomers . Nucleic Acids Res . ( suppl . ) 16 : r271 . Roberts , T.M. , S.L. Swanberg , A. Poteete , G. Riedel , and K. Backman . 1980. A plasmid cloning vehicle allowing a positive selection for inserted frag- ments . Gene 12 ...
Page
... isoschizomer pairs , 5.17 , 5.25 of restriction enzymes , 5.16-5.24 of type II DNA methylases , 5.25 modification of ... isoschizomers , 5.14 KGB buffer , 5.28-5.31 , B.26 ligation after cleavage , 5.10-5.13 partial digestion of high ...
... isoschizomer pairs , 5.17 , 5.25 of restriction enzymes , 5.16-5.24 of type II DNA methylases , 5.25 modification of ... isoschizomers , 5.14 KGB buffer , 5.28-5.31 , B.26 ligation after cleavage , 5.10-5.13 partial digestion of high ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml