Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 59
Page xxxvi
... Medium ( Luria - Bertani Medium ) A.1 NZCYM Medium A.1 NZYM Medium A.1 NZM Medium A.1 Terrific Broth A.2 SOB Medium A.2 SOC Medium A.2 2 × YT Medium A.3 M9 Minimal Medium A.3 MEDIA CONTAINING AGAR OR AGAROSE A.4 STORAGE MEDIA A.5 Stab ...
... Medium ( Luria - Bertani Medium ) A.1 NZCYM Medium A.1 NZYM Medium A.1 NZM Medium A.1 Terrific Broth A.2 SOB Medium A.2 SOC Medium A.2 2 × YT Medium A.3 M9 Minimal Medium A.3 MEDIA CONTAINING AGAR OR AGAROSE A.4 STORAGE MEDIA A.5 Stab ...
Page 1-33
... medium should be inoculated with a single bacterial colony or with a small liquid culture grown from a single colony . The appropriate antibiotic should be present in the medium . 2. Inoculate 500 ml of LB or Terrific Broth medium ...
... medium should be inoculated with a single bacterial colony or with a small liquid culture grown from a single colony . The appropriate antibiotic should be present in the medium . 2. Inoculate 500 ml of LB or Terrific Broth medium ...
Page 4-24
... medium into a sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick ...
... medium into a sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml