Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-23
... method can therefore be used with any strain of E. coli grown in cultures of any size . It is curious , however , that many laboratories use one method to process minipreps and another method to isolate plasmid DNA from large - scale ...
... method can therefore be used with any strain of E. coli grown in cultures of any size . It is curious , however , that many laboratories use one method to process minipreps and another method to isolate plasmid DNA from large - scale ...
Page 2-118
... method ( E.F. Fritsch , unpubl . ) and the liquid culture method ( Leder et al . 1977 ) . Both methods work equally well , although there are occasional problems with the yield and purity of the bacteriophage DNA . If trouble should ...
... method ( E.F. Fritsch , unpubl . ) and the liquid culture method ( Leder et al . 1977 ) . Both methods work equally well , although there are occasional problems with the yield and purity of the bacteriophage DNA . If trouble should ...
Page
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml