Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-57
... methylase . hsdS strains are defective in both restriction and methylation ( for a review , see Yuan 1981 ) . Bacterial gene encoding the subunit of E. coli methylase that methylates EcoK restriction sites . The EcoK restriction enzyme ...
... methylase . hsdS strains are defective in both restriction and methylation ( for a review , see Yuan 1981 ) . Bacterial gene encoding the subunit of E. coli methylase that methylates EcoK restriction sites . The EcoK restriction enzyme ...
Page 5-16
... methylase has been isolated that modifies the cognate recognition sequence and renders it resistant to cleavage . These methylases , a number of which are available from commercial suppliers , are useful in a number of tasks in ...
... methylase has been isolated that modifies the cognate recognition sequence and renders it resistant to cleavage . These methylases , a number of which are available from commercial suppliers , are useful in a number of tasks in ...
Page
... methylase , 2.57 , 5.15 dcm methylase , 5.15 deficient strains for growing bacterio- phage λ , 2.55 EcoK is missing from E. coli C , 2.99 , 5.15 EcoRI methylase , 5.16 treatment of cDNA , 8.24 , 8.66-8.67 hsdM , genetic marker in E ...
... methylase , 2.57 , 5.15 dcm methylase , 5.15 deficient strains for growing bacterio- phage λ , 2.55 EcoK is missing from E. coli C , 2.99 , 5.15 EcoRI methylase , 5.16 treatment of cDNA , 8.24 , 8.66-8.67 hsdM , genetic marker in E ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml