Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 68
Page 1-70
... mixture . Maximal stimula- tion is observed in ligation mixtures containing 15 % PEG 8000. Prepare reaction mixtures at 0 ° C by mixing all components except the PEG 8000 and the bacteriophage T4 DNA ligase . Then add an appropriate ...
... mixture . Maximal stimula- tion is observed in ligation mixtures containing 15 % PEG 8000. Prepare reaction mixtures at 0 ° C by mixing all components except the PEG 8000 and the bacteriophage T4 DNA ligase . Then add an appropriate ...
Page 2-94
... mixture . Optimum values for both of these parameters can be estimated from theoretical considerations ( see Chapter 1 , pages 1.63–1.67 ) . By necessity , however , such calculations assume that all the DNA molecules in the ligation ...
... mixture . Optimum values for both of these parameters can be estimated from theoretical considerations ( see Chapter 1 , pages 1.63–1.67 ) . By necessity , however , such calculations assume that all the DNA molecules in the ligation ...
Page 7-75
... mixture to a water bath set at the annealing temperature . Incubate the mixture for 8-12 hours . The optimal temperature for annealing varies from RNA to RNA , presumably depending on such factors as G + C content and propensity to form ...
... mixture to a water bath set at the annealing temperature . Incubate the mixture for 8-12 hours . The optimal temperature for annealing varies from RNA to RNA , presumably depending on such factors as G + C content and propensity to form ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
47 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml