Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 7-58
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. MAPPING RNA WITH NUCLEASE $ 1 Nuclease S1 is used • To map the location of 5 ′ and 3 ′ termini of mRNA on DNA templates • To locate the 5 ′ and 3 ′ splice junctions in ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. MAPPING RNA WITH NUCLEASE $ 1 Nuclease S1 is used • To map the location of 5 ′ and 3 ′ termini of mRNA on DNA templates • To locate the 5 ′ and 3 ′ splice junctions in ...
Page 7-60
... nuclease S1 ww exon 2 intron B exon 3 www Hybridize RNA to 5'- or 3 ' - labeled genomic DNA probes Analyze by gel ... nuclease S1 5 ' - labeled probe after digestion with nuclease S1 b * C = 3 ' - labeled probe after digestion with ...
... nuclease S1 ww exon 2 intron B exon 3 www Hybridize RNA to 5'- or 3 ' - labeled genomic DNA probes Analyze by gel ... nuclease S1 5 ' - labeled probe after digestion with nuclease S1 b * C = 3 ' - labeled probe after digestion with ...
Page 7-61
... nuclease by simultaneous hybridization to two different RNA molecules ( Lopata et al . 1985 ) . Fourth , nuclease S1 cleaves the segment of DNA opposite a looped - out region of RNA compara- tively inefficiently ( Sisodia et al . 1987 ) ...
... nuclease by simultaneous hybridization to two different RNA molecules ( Lopata et al . 1985 ) . Fourth , nuclease S1 cleaves the segment of DNA opposite a looped - out region of RNA compara- tively inefficiently ( Sisodia et al . 1987 ) ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml