Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-13
... nucleotides of each sequence are complementary , then three nucleotides can be added to the recessed termini , and the resulting partially repaired fragments can be ligated via the single remaining com- plementary nucleotide . If the ...
... nucleotides of each sequence are complementary , then three nucleotides can be added to the recessed termini , and the resulting partially repaired fragments can be ligated via the single remaining com- plementary nucleotide . If the ...
Page 5-74
... nucleotides from the 5 ' and 3 ' termini of double - stranded DNA is given by the equation : dM t = -2VM , n m dt [ Km + ( S ) , ] t n m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , V ...
... nucleotides from the 5 ' and 3 ' termini of double - stranded DNA is given by the equation : dM t = -2VM , n m dt [ Km + ( S ) , ] t n m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , V ...
Page 7-79
... nucleotides in length and are radiolabeled at both ends by phosphorylation or by incorporation of radiolabeled dNTPs at a recessed 3 ' terminus . Single - stranded DNA primers , which are preferred for experiments of this type , are ...
... nucleotides in length and are radiolabeled at both ends by phosphorylation or by incorporation of radiolabeled dNTPs at a recessed 3 ' terminus . Single - stranded DNA primers , which are preferred for experiments of this type , are ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml